TOLL樣受體基因多態(tài)性與胃癌及EBV相關胃癌易感性的研究.pdf

1、分類號：R373 密級：不保密UDC：578 學校代碼：11065碩 士 學 位 論 文TOLL 樣受體基因多態(tài)性與胃癌及 EBV 相關胃癌易感性的研究衛(wèi)美蓉 衛(wèi)美蓉指 導 教 師 王笑峰副教授學 科 專 業(yè) 名 稱 病原生物學論 文 答 辯 日 期 2016 年 6 月 1 日TLRs gene polymorphisms and susceptibility to gastric carcinomaand epstein-Barr

2、Virus-associated gastric carcinoma in northernAbstractObjective: The aim of our study was to evaluate the potential associations betweenthe SNPs of TLR genes and the risk of GC, especially to EBVaGC.Methods: The TLR2, TL

3、R4 and TLR9 genes polymorphism were assessed by poly-merase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 185cases of EBVnGC, 43 cases of EBVaGC. 100 cases of peripheral blood samples fromhealthy

4、individuals were also examined. And Genotyp of some samples wereanalyzed by PCR and sequencing. the data was analysed by SPSS.Results: As for the TLR2 gene rs3804099 and TLR4 gene rs10759932, there weresignificant differ

5、ences between the GC group and the control group in both genotypeand allelic frequency. As for TLR2 gene rs3804100 and TLR4 gene rs11536889, therewere no association between the GC group and control. As for TLR91486T/C(r

6、s187084) and C2848T(rs352140), there is also no association between the GC groupand control. In all the indicators, no polymorphisms were found to be related toEBVaGC in the studied population.Conclusions: The polymorphi

7、sms of TLR2 gene rs3804099 and TLR4 geners10759932 were associated with susceptibility to GC in Shandong Province of China.There was no interaction between EBV and TLR gene polymorphisms in EBVaGC.Graduate student: WEI M