雌、孕激素調控小鼠在體子宮內膜MEIS1表達的研究.pdf

1、華中科技大學碩士學位論文雌、孕激素調控小鼠在體子宮內膜MEIS1表達的研究姓名：李丹申請學位級別：碩士專業(yè)：婦產(chǎn)科學指導教師：章漢旺20090401華 中 科 技 大 學 碩 士 學 位 論 文華 中 科 技 大 學 碩 士 學 位 論 文 2Estrogen and progestin regulation expression of MEIS1 in mouse endometrium in vivo Abstract Ob

2、jective: Observation of the effects of estrogen and progesterone in mouse endometrium cells MEIS1 expression and analyze its relationship with endometrial receptivity. Methods: Randomly divide 48 mice which are 10 weeks

3、old 25~30g size Kunming female mice of clean grade into 4 groups. Separately for the control group (C) after castration injection 0.1ml vegetable oil ；estrogen group(E) after the injection of ovariectomized 17β-estradio

4、l (E2) 100ng ；Progesterone group (P) after castration injection progesterone (P) 1mg ；Estrogen + progesterone group (E + P) after castration injection E2100ng + P1mg ，each 12. Carried out using immunohistochemistry histo

5、logy positioning; reverse transcription polymerase chain reaction (RT-PCR) 、Real-Time PCR semi-quantitative, mRNA at the level of estrogen and progesterone on detection of mouse endometrial cells Meis1 gene expression. R

6、esult: Meis1 mainly expressed in the mouse endometrial epithelial and stromal cell nuclear membrane. Use of estrogen and progesterone alone or a combination of estrogen and progesterone to ovariectomized mouse endometriu

7、m Meis1 have a catalytic role (P <0.05), but the United's role in promoting is more obvious (P <0.01). Conclusion: Estrogen and progesterone to promote Meis1 gene expression in mouse endometrial cells , and es

8、trogen and progesterone peak of expression was significantly higher. Description Meis1 are endometrial important factor of the implantation window, its exposure to estrogen and progesterone synergy, then mediated a seri