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1、 人腦膠質細胞瘤基因啟動子超甲基化和組蛋 白修飾的臨床研究人腦膠質細胞瘤基因啟動子超甲基化和組蛋 白修飾的臨床研究 劉柏麟 培 養(yǎng) 類 別 全日制 學 位 類 型 學術學位 一級學科(專業(yè)類) 臨床醫(yī)學 二 級 學 科 ( 專 業(yè) ) 外科學（神外） 研 究 方 向 腦腫瘤的基礎與臨床研究 指 導 教 師 章翔教授（主任醫(yī)師） 培 養(yǎng) 單 位 西京醫(yī)院神經(jīng)外科 二 O 一三年五月 分 類 號 U D C 密 級 第 四

2、 軍 醫(yī) 大 學The Fourth Military Medical University博士學位論文第四軍醫(yī)大學博士學位論文 目 錄 縮略語表························

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9、·····5 ABSTRACT······························

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19、183;···17 正 文·······························

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22、;·················31 第一部分 惡性膠質細胞瘤多基因啟動子超甲基化及其預后意義··············&#

23、183;············31 1 材料·······················

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26、#183;······················32 1.1 膠質細胞瘤組織樣本 ···········

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31、·················32 1.3 主要儀器··················

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34、#183;············33 1.4 主要試劑······················

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37、183;········34 2 方法···························

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40、#183;··················34 2.1 DNA 提取 ···············

41、83;····································&

42、#183;····································

43、;··············34 2.2 DNA 甲基化免疫共沉淀（MeDIP） ··················

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45、3;·····37 2.3 Real-time PCR····························

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47、3;································40 2.4 統(tǒng)計學分析··&#

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53、83;··································43 3.1 膠質細胞瘤腫

54、瘤組織、血清和腦脊液 DNA 中的基因啟動子超甲基化 ···43 3.2 基因啟動子超甲基化與生存期的關系·························&#

55、183;·······························50 4 討論····

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58、#183;····································

59、;·····58 第二部分 膠質細胞瘤全局性組蛋白修飾及其預后意義 ···························

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