癌基因cjun在三氧化二砷抑制腺樣囊性癌細胞中的表達及意義

1、分類號：R777.5密級：不保密UDC：617學校代碼：11065碩士學位論文癌基因cjun在三氧化二砷抑制腺樣囊性癌細胞中的表達及意義癌基因cjun在三氧化二砷抑制腺樣囊性癌細胞中的表達及意義仝曉燕指導教師姜濤教授學科專業(yè)名稱眼科學論文答辯日期201565萬方數據ExpressionsignificanceofcjungeneinadenoidcysticarcinomacellsinducedbyArsenicTrioxideAbs

2、tractObjectiveThisexperimentaimstostudytheinvestigationfocusonexpressionofcjuninarsenictrioxideinhibitedproliferationinducedapoptosisofadenoidcysticcarcinoma2(ACC2)cellsinvitro.Thegoalistoexplefeasiblecluesontreatmentofa

3、denoidcysticcarcinoma.Methods(1)TheexperimentalgroupsweregivenvariousconcentrationofAs2O3whilecontrolgroupswithout.Mphologicalchangeswereobservedbyinvertedmicroscope.(2)RTPCRmethodwasusedtoinvestigatecjungeicchangesinmRN

4、Alevelinexperimentalcontrolgroups(3)ImmunohistochemicalmethodwasappliedtoinvestigateJunproteinexpressionafterAs2O3treatment.UsingSPSS19.0statisticalanalysispackagetocarryonthedataprocessinginspectionlevelfa=0.05.Results(

5、1)Microscopeobservation:ACC2cellsgrowthisslowadherentabilitydeclineinexperimentalgroupAs2O3caninhibittheproliferationofACC2cellinducecellapoptosiswhichwastimedependentconcentrationdependent.(2)RTPCR:Theresultsshowthatcju

6、nmRNAexpressthehighestamountinthecontrolgroup.WiththeincreaseofconcentrationofAs2O3culturingtimetheexpressionofcjunmRNAdecreasedobviouslyreachitsbottomin6molLconcertrationat48h.Thedifferencewasstatisticallysignificant(P=

7、0.001).(3)Immunohistochemistry:TheresultsshowJunproteinexpressioninthenucleuswithbrownyellowbrownnotincytoplasm.JunproteinishighlyexpressedinACC2cellsitsexpressiondecreasedinACC2celltreatedwithAs2O3inatimedosedependentma

8、nner.Thedifferencewasstatisticallysignificant(P=0.008).Conclusion(1)As2O3caninhibitthegrowthofACC2cellsinvitroinducecellapoptosis(2)TheexpressionofcjunmRNAproteinhasobviousspecificityinACC2cellsinvitroAs2O3canchangethele

9、velofexpressionofcjunmRNAproteinlevelwhichwastimedependentconcentrationdependent(3)cjungeneplayanimptantroleintheapoptosisofACC2cellinducedbyAs2O3whichmayprovideagoodbaseontheyfclinicalapplicationofAs2O3onACC.Postgraduat